spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Mapping the gene-trap insertion in the Man1 locus. (A) Upper panel: the Man1 gene, showing exon organization (black boxes). Lower panel: the gene-trap vector, containing the engrailed2 intron (en2) and splice acceptor (SA), a ß-galactosidase-neomycin fusion cDNA (ß-geo), an SV40 polyadenylation site (SV40pA) integrated between exons 4 and 5. `Probe' indicates the location of the genomic probe used in Southern blotting. (B) Southern blot of genomic DNA from gene-trapped mice. Approximate size of detected DNA is indicated. (C) Northern blot, demonstrating 4.7 kb endogenous and 7.7 kb Man1/ß-geo transcripts in wild-type (+/+) or heterozygous (+/GT) MEFs. (D) The MAN1 911 amino acid polypeptide contains the LEM domain at the N-terminus, the RRM domain at the C-terminus and two transmembrane domains. The gene-trap insertion product retains the 567 N-terminal amino acids (arrow) linked to ß-galactosidase. (E) Western analysis on MEF whole cell extracts using an antibody raised to a C-terminus peptide shows that the C-terminus of MAN1 is absent in homozygotes (GT/GT). +/+, wild-type; +/GT, heterozygote; E, EcoRV.





Right arrow Return to article