DEV02822 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Experiments showing that DiI and DiO diffusion is absent, and that central and peripheral parts of the limb bud have similar prospective fate along the proximal-distal axis. (A-C) DiI and DiO do not diffuse freely within 2 days of administration. After DiI and DiO administration at stage 22 (A), the embryo was fixed and then left on a dish for 2 days at 4°C. Neither sample showed any leaky diffusion 1 day (B) or 2 days (C) after administration. (D-H) Central and peripheral parts of the limb bud have similar prospective fate along the proximal-distal axis. (D) Limb bud immediately after labeling with DiI. (E) Contribution of labeled cells after 2 days. (F-H) Longitudinal section of the same limb bud. The limb bud is oriented with dorsal to the top and distal to the right. Fluorescence for DiI was observed in a straight belt along the dorsal-ventral axis.

• Supplemental Figure 2 -

  Fig. S2. Quantitative RT-PCR analysis of hoxa-11/a-13 transcripts in the distal limb bud at various stages. The relative levels of hoxa-11 (A) and hoxa-13 (B) expression were plotted after being standardized with that of β-actin. The amount of transcripts at stage 20 was taken as 1.0, and the relative amount is shown on each bar. Each experiment was repeated four times. Error bars indicate s.d.