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Figure 2


Fig. 2. Specificity of P2X primer and probe sets and standard curve analysis. (A) Real-time PCR amplification plot showing specificity of P2X receptor primer/probe sets with their target templates. Seven successful amplifications are shown, with absence of amplification of mismatched templates (see B). (B) Grid representation of primer and probe specificity showing CT values for on-target cDNA amplification for all seven P2X receptors. Note the absence of non-specific amplification. (C) An example of a standard curve for P2X1 receptor cDNA amplification. Note the linearity to <10 copies. (D) Standard curve analysis of the P2X receptor targets showing linearity of all seven dilution series. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NSE, neuron-specific enolase. Copy range used for the standard curves is estimated from the serial dilutions of template cDNAs.





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