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Figure 4


Fig. 4. Variation in expression of P2X2 splice variants in SGN. (A) Schematic of the three P2X2 isoforms expressed in SGN, showing their C-terminal region variation. ECD, extracellular domain; TM, transmembrane domain. (B) End-point single-cell RT-PCR analysis of a sample of six SGN showing the expression of P2X2 isoforms in individual cells (lanes 1-6). Amplicon sizes: P2X2-1=499 bp; P2X2-2=292 bp; P2X2-3=480 bp. P2X2-3 was the most prominent isoform (4/6 cells). The agarose gel also includes two positive controls of cDNA from whole spiral ganglion (lane 9) and whole cochlea (lane 10). Lane 7 (-) control for no template. Lane 8 (-) bath sample processed for RT-PCR. (C) Analysis of P2X2 splice variant combinations detected in the 31 single-cell RT-PCR experiments. P2X2-3 was the dominant isoform either expressed alone or with one or more of the other isoforms (23/31 neurons).





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