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Fig. 3. Trafficking of Yolkless and Gurken is impaired in rab6^D23D oocytes. Egg chambers stained for Yolkless (A,B, green) or Gurken (C-E, green), F-actin (phalloidin, red) and DNA (DAPI, blue). In wild type (A), Yolkless concentrates in the oocyte cytoplasm before stage 8 (arrowhead). At stage 10, Yolkless is restricted to the oocyte membrane (A'). In rab6^D23D egg chambers (B), Yolkless concentrates in the oocyte normally (arrowhead). However, at stage 10, an important proportion of Yolkless remains cytoplasmic, indicating that trafficking is impaired (B'). In wild type (C), Gurken is detected as a small arc between the oocyte nucleus and the antero-dorsal plasma membrane. Secreted Gurken is detected in the cytoplasm of the overlying follicle cells (C'', arrow). In the majority of affected rab6^D23D stage-10 egg chambers (55.5%), large amounts of ectopic Gurken protein are detected in the ooplasm (D,E). In some (29.8%) affected rab6^D23D egg chambers, secreted Gurken is still detected in the cytoplasm of the overlying follicle cells (D'', arrow). In others (25.7%), Gurken is detected exclusively in the ooplasm and is not detected in the follicle cell cytoplasm (E'').

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