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Figure 4


Fig. 4. Sec5 and Syx1A localization on membranes is differentially impaired in rab6 egg chambers. Egg chambers stained to reveal Syx1A (A,B, green) or Sec5 (C,D, green), F-actin (phalloidin, red) and DNA (DAPI, blue). In wild type (A), Syx1A is detected on nurse cell and oocyte membranes (arrows). After stage 9, Syx1A is predominantly detected on nurse cell membranes. In rab6D23D egg chambers (B), Syx1A is detected on the remaining plasma membranes (arrow) and around the ring canal cluster (arrowheads), in both strongly and in mildly affected egg chambers. In stage-8 wild-type egg chambers (C), Sec5 and actin colocalize at nurse cell (arrow) and oocyte (Oo) plasma membranes. In rab6D23D egg chambers at these stages, Sec5 is absent from residual membranes that delimit nurse cell `open' syncytia (D, arrow) but is readily detected on the oocyte plasma membrane (Oo). Sec5 is not detected in the aggregates of ring canals and actin debris (D, arrowhead).





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