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Fig. 4. Shroom3 controls the distribution of 
-tubulin in
neuroepithelial cells during neural tube closure. (A)
High-magnification projection of a stack of optical sections showing
elongating neural epithelial cells stained for -tubulin (white lines
indicate cell outline). (a') 
-tubulin staining for cells
shown in A. (B) Schematic of the closing neural tube indicating
location of section shown in A (red box). (C) -tubulin staining
in a projection of optical sections reveals centrosome-like foci at the apical
surfaces of mature neuroepithelial cells. (c')
Acetylated--tubulin staining reveals cell outlines in the ventral
region of the closed Xenopus neural tube. (D) Schematic of the
neural tube indicating location of section shown in C (red box). (E)
Optical section showing -tubulin in an embryo unilaterally injected
with Shroom3-MO (right side is injected, left side is control).
(e') -tubulin staining for cells shown in E.
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