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Fig. 6. Shroom3 is sufficient to drive apical accumulation of ${gamma}$ -tubulin in naïve epithelial cells. (A) Control embryo showing surface view of the superficial blastomere epithelium stained with anti- ${gamma}$ -tubulin. (B) Shroom3-expressing embryo showing surface view of the superficial blastomere epithelium stained with anti- ${gamma}$ -tubulin. (C) Cross-section through superficial blastomeres of a control embryo at early stages stained with anti- ${gamma}$ -tubulin. Merged image is shown in c' (green, ${gamma}$ -tubulin; red, propidium iodide). (D) Cross-section through superficial blastomeres of an embryo ectopically expressing Shroom3 at early stages stained for anti- ${gamma}$ -tubulin. Merged image is shown in d' (green, ${gamma}$ -tubulin; red, propidium iodide). (E) Cross-section through superficial blastomeres of an embryo ectopically expressing Shroom3 at later stages. Embryo has been stained to detect Myc-Shroom3 (E; red) and ${gamma}$ -tubulin (e'; green). Merged image is shown in e''.

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