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Figure 7


Fig. 7. Expression of Shroom3 in epidermal cells recapitulates the events underlying neuroepithelial cell shape change. (A) Control epidermis stained for {alpha}-tubulin. (B) Epidermis expressing Shroom3 stained for {alpha}-tubulin. Cells expressing Shroom3 (marked by anti-Myc; b') constrict and elongate. Parallel arrays of MTs emanate from the apical cell surface. (C) Control epidermis stained for {gamma}-tubulin. (D) Epidermis expressing Shroom3 stained for {gamma}-tubulin. Cells expressing Shroom3 in this panel (marked by anti-Myc, red; d') are only slightly constricted, but are elongated and contain a broad, diffuse accumulation of {gamma}-tubulin. (E) A single epidermis cell expressing high levels of Shroom3 apically constricts and also elongates. (e') Anti-{alpha}-tubulin staining of the cell shown in E.





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