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Figure 8


Fig. 8. Shroom3 coordinates the polarized assembly of both actin and {gamma}-tubulin cytoskeletons. (A) Control embryo at stage 6 showing surface view of the superficial blastomere epithelium stained with phalloidin (a') and anti-{gamma}-tubulin (a''). (B) Shroom3-expressing embryo at stage 6 showing surface view of the superficial blastomere epithelium stained with phalloidin (b') and anti-{gamma}-tubulin (b''). (C) Control embryo at stage 7.5 showing surface view of the superficial blastomere epithelium stained with phalloidin (c') and anti-{gamma}-tubulin (c''). (D) Shroom3-expressing embryo at stage 7.5 showing surface view of the superficial blastomere epithelium stained with phalloidin (d') and anti-{gamma}-tubulin (d''). Bracket indicates accumulated {gamma}-tubulin with little actin; arrow indicates accumulated actin with little {gamma}-tubulin.





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