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Figure 1


Fig. 1. Follicle cell clones mutant for papss, sgl or sfl are viable even when the germline is also homozygous for the same mutation. Follicle cell and germline nuclei in stage-10 egg chambers visualized by DAPI (A,C,E,G,I) and GFP (B,D,F,H,J) fluorescence. Nuclei of mutant cells lack the GFP marker but still exhibit DAPI fluorescence. (A,B) Egg chamber from e22c-GAL4, UAS-FLP/+; hGFP FRT79D/papss2 FRT79D female containing papss mutant cells in the follicular epithelium. (C,D) Follicle from hsFLP1/+; hGFP FRT79D/papss2 FRT79D female with papss mutant germline cells. (E-J) Follicles carrying both follicle cell and germline mutant clones from females of the following genotypes: (E,F) hsFLP1/+; e22c-GAL4, UAS-FLP/+; hGFP FRT79D/papss2 FRT79D; (G,H) hsFLP1/+; e22c-GAL4, UAS-FLP/+; hGFP FRT79D/sgl08310 FRT79D; (I,J) hsFLP1/+; e22c-GAL4, UAS-FLP/+; hGFP FRT79D/sfl03844 FRT79D.





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