Supplemental Figure 1
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Fig. S1. Expression of Pumilio or Nanos was not disrupted in stwl<b>− ovaries or when Stwl was ectopically expressed. (A-C) Pumilio (Pum) expression (green) in early germ cells also labeled with anti-hts (red) and Hoechst (blue). (A) In wild type, Pum was expressed highly in stem cells and cystoblasts. (B) When Stwl was ectopically expressed, Pum expression pattern was not significantly altered. (C) In stwl mutant animals, Pum was not reduced, but rather was expressed in slightly more cells than wild type. (D-F) Expression of a Myc-tagged Nos-rescuing transgene. (D) In wild type, Nos (green) was expressed highly in stem cells and cystoblasts, and again in 16 cell cysts. (E) When Stwl was ectopically expressed, Nos expression pattern was not significantly altered. (F) In stwl mutant animals, Nos expression was not reduced.
