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Fig. 6. Ephrin-Ad acts upon the notochord/neural mother cells. (A)
Experimental strategy. Eggs were injected with ephrin-Ad-MO, fertilised and
cultured until the late 32-cell stage, when the notochord/neural mother cells
were isolated. The isolated mother cells were further cultured until the
equivalent of the early gastrula stage, when they were fixed for in situ
hybridisation analyses for the expression of Ci-Bra and
Ci-ETR. At this stage, the resultant partial embryos consist of four
cells. (B) Histograms showing the average numbers of cells positive for
Ci-Bra (magenta) and Ci-ETR (blue) in partial embryos as
indicated on the left of the graphs. The data are expressed as
mean±s.e.m. Significant differences compared to the results obtained
from the control were evaluated by the Student's t-test and indicated
with asterisks (P
0.01). n, total number of samples
analysed.
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