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Fig. 2. Effect of endoderm transplantation. (A,B) Diagrams of
the experimental design for endoderm transplantation. Ectoderm cells were
removed from early 32-cell host embryos (red twisted arrows). Donor endoderm
cells were isolated from early 32-cell embryos. The isolated endoderm cells
were transplanted to the ectoderm-depleted region (red arrowheads) so that the
presumptive mesenchyme (A) and notochord (B) cells are sandwiched with two
groups of endoderm cells. (C-N) Expression of tissue-specific markers
in un-manipulated control (left column), endoderm-transplanted (middle column)
and ectoderm-transplanted control (right column) embryos. (C-K) Vegetal views.
(L-N) Animal views. Anterior is up. (C-E) Expression of the mesenchymespecific
Mch-3 antigen in embryos in which cleavage was arrested at the 110-cell stage
with cytochalasin B. Arrowhead in D indicates a muscle blastomere that
ectopically expressed the Mch-3 antigen. (F-H) Expression of the muscle
actin gene at the 110-cell stage. (I-K) Expression of
notochord-specific Hr-Bra at the 110-cell stage. Arrowheads in J
indicate cells of nerve cord lineage that ectopically expressed
Hr-Bra. (L-N) Expression of neural plate-specific Hr-ETR1 in
cleavage-arrested 110-cell embryos. Two rows of expression were observed in
brain-lineage (arrowheads b) and nerve cord-lineage (arrowheads n) cells. The
percentages at the bottom of each photo represent the proportion of embryos
that ectopically expressed the Mch-3 antigen and Hr-Bra, and that
showed reduced expression of actin and ETR1. Scale bar: 100
µm.
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