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Fig. 5. Suppression of notochord fate by a signal from ectoderm.
(A-B') Expression of Hr-Bra in 110-cell embryos (A,B;
vegetal views) and of ETR-1 in cleavage-arrested 110-cell embryos
(A',B'; animal views). Anterior is up. (A,A') Embryos with
a-line cells removed at the early 32-cell stage. Arrowheads indicate cells in
the nerve cord lineage that ectopically expressed Bra. (B,B')
Embryos with a-line cells removed at the early 64-cell stage.
(C-E') Expression of Hr-Bra and ETR-1 in
partial embryos. (C,C') Every a-line cell (Ecto) and nerve
cord/notochord precursor was co-isolated from early 32-cell embryos and
cultured until the 110-cell stage. Arrowheads in C' indicate cells of
notochord lineage. They did not express ETR1. (D,D',E,E')
The same co-isolate as in C was recombined at the notochord side with a-line
cells (D,D') or mesenchyme precursors (E,E'). In D', four
rows of ETR1-expressing cells were observed, consisting of two rows
of notochord and nerve cord precursors flanked by two rows of brain cells. Br,
brain; Epi, epidermis; Mes, mesenchyme; NC, nerve cord; Not, notochord
precursors. Scale bar: 100 µm.
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