QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Deleterious consequences of Nos-dependent repression of CycB mRNA outside the PGCs. (A) Darkfield photomicrographs of cuticle secreted by embryos from wild-type (wt) or CycB²/CycB³; CycB(2x hbNRE) females. Note the reduction of abdominal segments, each marked by a band of large denticles (e.g. black arrowhead) and the rudimentary posterior terminalia (filzkörper, white arrowhead); both features are absent in the limit CycB (2x hb NRE) phenotype (above). (B) Surface views of nuclei in two different cycle 10-13 embryos from females of the indicated genotype. The wild-type embryos are from CycB²/+ females. Note that many nuclei in the posterior of the embryo at the lower right have fallen into the center and are not visible in the focal plane shown. (C) Distributions of CycB (green) and Vasa (red) in mid-line views of embryos from females of the indicated genotype. The posterior region that contains lower levels of CycB is bracketed. To the right are high magnification views of post-cycle 9 embryos, showing repression of CycB (2x hbNRE) in the PGCs. The white arrowhead highlights nuclei that have fallen into the interior. The embryo on the left is from a CycB²/+ female and the embryo in the middle from a CycB²/+ female bearing the CycB (I+II) transgene.

Return to article