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Fig. 2. Dvir rho4.2 carries a minimal enhancer activity accounting
for the endogenous rho expression pattern during DA formation.
(A,B) D. virilis rho mRNA was detected in a V-shaped
stripe missing its apex at stage 10B (A), and at stage 12 its distribution was
restricted to the four domains where the four DAs later form (B).
(C,D) The Dvir rho4.2 enhancer drove the expression
of GFP in a pattern similar to that of the endogenous rho in D.
virilis. At stage 10B, a Vshaped stripe missing its apex was observed
(C), and then at stage 12, the GFP reporter was expressed where the four DAs
would later form (D). White arrowheads and brackets indicate the cells forming
the anterior and posterior DAs, respectively (B,D). (E-G) Confocal
images of the GFP reporter expression driven by the Dvir rho4.2
enhancer and of endogenous Broad-Complex (BR-C) expression. The expression of
Dvir rho4.2-GFP (green in E and G) and BR-C (magenta in F and G)
was mutually exclusive at stage 10B. G is a merged image of E and F.
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