(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. Function of the rho enhancer also diverged between
D. melanogaster and D.
virilis. (A-I) D. melanogaster transgenic
flies carrying both Dmel rho2.2-ß-Gal (magenta in A,C,D,F,G,I)
and Dvir rho4.2-GFP (green in B,C,E,F,H,I) reporters were
generated. The activities of the two enhancers were then determined at
different developmental stages: early in stage 10B (A-C), late in stage 10B
(D-F) and stage 12 (G-I). (A-C) At early stage 10B, Dmel
rho2.2-ß-Gal (A) was activated, but Dvir rho4.2-GFP
(B) was not yet active (C). (D-F) Late in stage 10B, Dvir
rho4.2-GFP expression began (E) in a pattern similar to that of
Dmel rho2.2-ß-Gal (D). The domain of Dvir
rho4.2-GFP activation (E) largely overlapped with that of Dmel
rho2.2-ß-Gal (D), although a slight posterior expansion of
Dvir rho4.2-GFP was seen (F). (G-I) The anterior boundaries of the
Dmel rho2.2-ß-Gal (G) and Dvir rho4.2-GFP (H)
activation domains were similar, but the posterior expansion of Dvir
rho4.2-GFP expression became prominent (I). The right panels are
merged images of the left and middle panels.