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Figure 5


Fig. 5. Conservation of the Grk signal in D. melanogaster and D. virilis. (A) The experimental scheme for interspecific pole cell transplantation from D. virilis to D. melanogaster embryos. (Top) Pole cells were transplanted from D. virilis donor embryos into D. melanogaster host embryos carrying the EGFP-vasa construct. (Bottom) Left: a normal egg chamber composed of an EGFP-Vasapositive D. melanogaster oocyte and D. melanogaster somatic follicle cells. The D. melanogaster Grk activated D. melanogaster EGFR in this egg chamber. Right: a chimeric egg chamber. In the egg chamber of a D. melanogaster host female, the oocyte was replaced with one of D. virilis origin. In this chimeric egg chamber, the D. virilis Grk activated the D. melanogaster EGFR and directed the DA formation. (B-B'') A gonad of a D. melanogaster host embryo with transplanted D. virilis pole cells. The host embryos were stained with anti-GFP (B, green) and anti-Vasa (B', magenta) antibodies. The D. melanogaster host pole cells expressed both GFP and Vasa (B, green; B'', white), whereas the transplanted D. virilis pole cells expressed only Vasa (B',B'', magenta). The D. virilis pole cells were located within the gonad of the D. melanogaster host (B'', magenta). B'' is a merged image of B and B'. (C) Ovary of a D. melanogaster host female. GFP-positive host germline (green) and GFPnegative D. virilis germline (white arrowhead) cells are shown. The D. virilis germline was surrounded by D. melanogaster host follicle cells. (D,E) The chimeric egg (negative for EGFP, data not shown) had two DAs (E) identical to those of the wild-type egg (D). (F,G) The percentages of eggs in which the DA roots were separated by the distances indicated in the horizontal axes are shown. The two DAs were separated by a mean distance of 48.2±4.6 µm (n=57) in the wild-type (F) and 49.2±3.6 µm (n=12) in the chimeric (G) eggshells. The differences were not significant (0.5>P>0.2, Student's t-test).





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