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Fig. 5. Rescue of optic stalk defects in Fak56D mutants by SG-cell-specific expression of a Fak56D transgene. (A-C) Expression of Gal4 lines used for rescue experiments, visualized with UAS-mCD8GFP (green). (D-F) Fak56D optic stalks carrying NP2109-Gal4, UAS-Fak56D (D) or NP4702-Gal4, UAS-Fak56D (E) or GMR-Gal4, UAS-Fak56D (F). Arrows indicate optic stalks (white, visualized with anti-Perlecan). (G) Quantification of optic stalk defects in animals carrying constructs as described. Expression of exogenous Fak56D in SG cells significantly rescued optic stalk disruption, while photoreceptor cell expression of Fak56D did not rescue the phenotype. Note that rescued optic stalks with NP4702-Gal4 exhibit the thinner morphology than those of wild type (asterisk). (H) Fak56D overexpression in wild type. Columns represent mean length of optic stalks carrying NP4702-Gal4 (white column) or NP4702-Gal4, UAS-Fak56D (gray column). Fak56D overexpression resulted in thinner optic stalks (^***P>0.001), while length of the optic stalks did not change (P=0.73). The error bars indicate s.d. (A-F) Anterior up, dorsal right. Genotypes for rescue: Fak56D^CG1/Fak56D^CG1; NP2109/+, Fak56D^CG1/Fak56D^CG1; NP2109/UAS-Fak56D, Fak56D^CG1/Fak56D^CG1; NP4702/+, Fak56D^CG1/Fak56D^CG1; NP4702/UAS-Fak56D, Fak56D^CG1/Fak56D^CG1; GMR-Gal4/+, Fak56D^CG1/Fak56D^CG1; GMR-Gal4/UAS-Fak56D. Genotypes for Fak56D overexpression: NP4702/+, NP4702/UAS-Fak56D.

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