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Fig. 6. SG cells fail to be organized into a tubular structure in
Fak56D mutants. (A,B) A wild-type (A) and a
Fak56D mutant (B) optic stalk at late third instar larval stage were
stained with anti-
-Tubulin (white). In a wild-type optic stalk, SG
cells express a high level of -Tubulin (white, anti--Tubulin)
(arrow in A). In a Fak56D mutant, SG cells failed to be organized
into a tubular structure and instead spread over the surface of the optic lobe
(arrow in B). (C-D') A wild-type (C) or a Fak56D mutant
(D) optic stalk of an early third instar larva carrying
ombP1 (white, visualized with anti-ß-gal).
The BM is visualized with anti-Perlecan (green). Anti-HRP (magenta) stained
only Bolwig's nerves. In a Fak56D mutant at the early third instar,
SG cells spread over the surface of the optic lobe (arrow in D).
(C',D') Glial cells are shown alone in these panels. The outlines
of the optic stalks are indicated with dashed yellow lines. (E-G)
Subsets of SG cells were clonally labeled with UAS-mCD8GFP (green) in a wild
type (E) or Fak56D mutants (F,G). SG cells in the optic stalk were
visualized with anti--Tubulin (white). SG cells distributed along the
AP axis and made contacts with each other via their processes (arrowhead in
E). In Fak56D mutants, SG cells exhibited normal elongated morphology
(arrowheads in F) but occasionally failed to distribute properly (arrows in
G). (H) Quantification of SG cell distribution along the AP axis in
wild type (yw) and Fak56D mutants. Positions of SG cells in clones
containing a few cells were classified into four groups.
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