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Figure 7


Fig. 7. Identification of CdGAPr as a gene involved in optic stalk morphogenesis. (A) Expression of NP3053-Gal4, which is inserted in the first intron of the CdGAPr, is visualized with UAS-mCD8GFP (green). NP3053-Gal4 exhibits restricted expression in SG cells (arrow) and WG cells (arrowhead). R axons are visualized with anti-HRP (magenta). (B) CdGAPr gene organization, showing the positions of P-element insertions in the CdGAPNP3053 and CdGAPr EY13451 lines. (C,D) Optic stalk morphology in a wild type (C) and a CdGAPrNP3053 homozygote (D), visualized with anti-Perlecan (magenta). A CdGAPrNP3053 homozygote exhibited optic stalk disruption (D). Arrows indicate optic stalks. (E) Quantification of optic stalk defects in various allelic combinations of CdGAPr alleles. (F) Quantification of optic stalk defects in animals carrying NP3053-Gal4, UAS-CdGAPr-dsRNA. (G) Quantification of the optic stalk defects in trans-heterozygous combinations of Fak56D and CdGAPr alleles, or trans-heterozygous mutants for Fak56D and myospheroid (mys). (A,C,D) Anterior up, dorsal right.





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