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Fig. 2. The tombola region and analysis of predicted protein
sequence. (A) tombola genomic region adapted from FlyBase.
The tomb 5' UTR is 130-191 bp long; the 3' UTR is 71 bp
long. Black boxes, coding sequence; grey boxes, UTRs; inverted triangles,
insertion sites of P[EY]00456 (EY) and P[GS]12862 (GS).
(B) Predicted sequence of Tomb protein. Thick underline, predicted
nuclear localisation signal; bold, CXC region; light grey box,
P[GS]12862 insertion site; thin underline, C-terminal conserved
region. (C) Alignments of CXC domains and spacer from tomb;
Drosophila mip120; human (hs) tesmin (tes) and tesmin-like (tesl);
Ciona intestinalis (Ciona); Strongylocentrotus
purpuratus (S. purp); C. elegans LIN-54;
Arabidopsis (At) TSO1 and SOL2. Con(1) and Con(2)
indicate amino acids in the first and second CXC domains: *,
conserved Cys; =, residues conserved in both CXC domains; 
,
residues conserved within CXC(1) or CXC(2), but which differ between the
domains. The E(z) Cys-rich region is shown as an outgroup. (D)
Alignment and predicted secondary structure (beneath) of the animal
tesmin-family protein C-termini. Predictions of secondary structure are shown
in the same order as the sequences (i.e. the first line is the Tomb secondary
structure prediction). Black lines indicate high confidence helix predictions;
the intervening region (grey line) has either no strong structural prediction
(Tomb), or a strong coil prediction (Mip120, Hs-tes, Hs-tesl).
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