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Fig. 6. tomb is more like aly than topi. (A-D) Hoechst 33342 labelling of primary spermatocyte DNA in live squashes and (A'-D') corresponding phase contrast images. In wild type (A,A'), the three major bivalents are decondensed, adjacent to the nuclear envelope. Chromosomes in aly (B,B') mutant primary spermatocytes are apposed to the nuclear envelope, but fuzzier and less well defined than in the wild type. Chromosomes in topi (C,C') mutant primary spermatocytes are partially condensed, and not close to the nuclear envelope. (D,D') tomb mutant primary spermatocyte chromosomes resemble those in aly mutants rather than those in wild type or topi mutants. (E-P) RNA in situ hybridisations. CG3330 (E,H,K,N), CG12907 (F,I,L,O) and CG3927 (G,J,M,P) in wild type (E-G), aly (H-J), topi (K-M) and tomb^GS12862 (N-P). In wild type, CG3330 message (E) persisted from primary spermatocytes until mid-elongation spermatids. CG3330 transcript was undetectable in topi testes (K), whereas aly and tomb testes had low levels of transcript (H,N). CG12907 was expressed in wild-type primary spermatocytes (F) and persisted to late elongation. This transcript was not detected in topi mutant testes (L); levels in aly and tomb spermatocytes (I,N) were similar to wild type. CG3927 in wild type was detected only in primary spermatocytes (G). aly and tomb testes showed robust expression of this gene (J,P), whereas topi testes showed low levels of CG3927 transcript (M).

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