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Fig. 3. The targeting strategy used for the Ripply2 gene
and the external morphology of the resulting knockout mouse. (A)
The top line shows the genomic organization of the Ripply2 gene, the
second line represents the structure of the targeting vector, and the bottom
two lines show the predicted structure of the Ripply2 locus following
homologous recombination. The first exon of Ripply2 was partially
deleted and replaced with a floxed neo cassette (the arrowheads on
the line represent loxP sites). A germline chimeric mouse was then generated
from recombinant ES cells containing the targeted allele and crossed with a
CAG-Cre mouse to remove the neo cassette and establish the
Ripply2-knockout mouse line. Ssp, SspI; E, EcoRI;
B, BamHI; H, HindIII; N, NcoI; K, KpnI; X,
XhoI. (B) The Ripply2-null mouse dies soon after
birth and the external morphology at E17.5 is similar to those of
segmentation-defective mutants, featuring a short trunk with rudimental
tails.
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