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Figure 6


Fig. 6. Notch signaling is reduced in the anterior PSM in the Ripply2-/- embryo. (A-F) Notch1 mRNA (A, n=2; B, n=2), Notch1 protein (C, n=2; D, n=2) and Hes5 mRNA (E, n=2; F, n=4) expression patterns were compared between wild-type (A,C,E) and Ripply2-/- (B,D,F) embryos at E11.0. (G-I) Double immunostaining with anti-Mesp2 (green) and anti-active Notch1 (magenta; the white lines indicate activities in the anterior PSM) antibodies using sections of wild-type (G) and Ripply2-/- (H,I) E11.0 embryos. In the Ripply2-/- background, Mesp2 expression is upregulated but Notch activity is reduced.





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