DEV001750 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Effect of mosaic deletion of Mi-2β in ventral epidermis. Hematoxylin-Eeosin-stained cross-sections of mutant skin at P1. (A) The black lines demarcate examples of islands of multilayered epidermis occasionally found in skin exhibiting the more severe ventral phenotype. (B) Magnified view of the boxed region in A. Black bracket identifies multilayered epidermis, red brackets indicate the prevalent epidermal depletion phenotype. Note the basophilic basal keratinocytes (dark purple) present beneath a substantial suprabasal epidermis and stratum corneum (pink) in the black-bracketed region. By contrast, basophilic basal keratinocytes and suprabasal epidermis are absent in the red-bracketed region where the stratum corneum abuts the dermis.

• Supplemental Figure 2 -

  Fig. S2. Expression of hair follicle markers and Mi-2β and Mi-2α in WT and mutant skin. (A) Semi-quantitative RT-PCR for hair follicle markers in P1 dorsal epidermis. RT-PCR was performed with primers specific for downless (Edar), β-catenin, Lef1, Bmp2, Shh, Patched1, Mi-2β (E11-13, within the deleted region), Mi-2β (E30-34, downstream of the deleted region) and Mi-2α RNA, which was prepared from P1 dorsal epidermis. Amplification of GAPDH is shown as a loading control. The dilution of cDNA used for each primer set is indicated at the lower right-hand corner of the panel. Two different WT samples are included in this analysis. (B) In situ hybridization studies reveal Mi-2β and Mi-2α mRNA expression in E18.5 skin. No increase in the normally low level of Mi-2α expression was detected in the mutant skin.