DEV02824 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Whole-mount and sagittal section comparison of Cre expression. (A-E′) E10.5 R26R detection reveals that Nkx2.5^Cre encompasses AHF and endocardium (A,A′), Wnt1-Cre: NCC (B,B′) AHF-Cre: AHF and derivatives (C,C′), TnT-Cre: specified myocardium (D,D′) and Tie2-Cre: endothelial cells (E,E′).

• Supplemental Figure 2 -

  Fig. S2. Smo is not required in myocardium or in endocardium for OFT development. (A-C) Tissue-specific elimination of Smo in either Tie2-Cre domain (B) or TnT-Cre domain (C) results in grossly normal OFT septation (A). (D) Shh expression is not detectable at 30 cycles (top row) and is barely detectable in some cardiac samples by RT-PCR between E9.5 and E12.5 (in duplicate).