|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. curly up affects expression of left-specific genes in the
lateral plate mesoderm (LPM) and dorsal diencephalon. Dorsal views of
spaw (A-D) and lefty1/2 (E-H) expression in
20- to 22-somite stage embryos (A-H) and ventral views of pitx2
expression in the dorsal diencephalon of 24-somite stage embryos (I-L)
from cup heterozygote crosses. Alterations from normal left
asymmetric Nodal signaling in the LPM (A) in cup mutants include
bilateral (B), right (C), and absent (D) expression of spaw in this
tissue. Over half of the bilaterally expressing embryos show only posterior
propagation of spaw. Similarly, asymmetrically expressed downstream
targets of Nodal signaling in the LPM and brain are also disrupted.
lefty1/2, which are normally expressed in the left cardiac LPM (E),
have bilateral (F), right (G), and absent (H) expression patterns in
cup mutants. In H, midline expression of lefty1 can also be
seen. pitx2, which is normally expressed on the left side of the
diencephalon (I), can be expressed bilaterally (J), on the right (K), or not
at all (L) in cup embryos. The majority of embryos show no expression
of lefty1/2 in the LPM and diencephalon and no expression of
pitx2 in the diencephalon.
|
