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Fig. 2. Expression of inhibitory HLH factors in differentiating astrocytes.
(Aa-Ag) Expression of Id1 and Hes1 in S100ß+ astrocyte
at E16.5. Arrowheads in Aa-Ac indicate the loss of expression of Pax6, Olig2,
and Nkx2.2 in some Id1+ cells in the VZ (higher magnification views
in insets), whereas those in Ad-Ag indicate the co-expression of Id1 and Hes1
with Pax7 and S100ß. (Ba-Be) Segregation of GFAP+ cells
with Pax6+, Olig2+, and Nkx2.2+ cells at
E18.5. Arrowheads indicate no overlap of GFAP+ cells with
Pax6+, Olig2+ and Nkx2.2+ cells.
(Bf-Bj) Expression of Id1 and Hes1 in GFAP+ and
S100ß+ astrocytes at E18.5. Arrowheads in Bf-Bj show
co-expression of GFAP and S100ß with Id1 and Hes1. Bc,Be,Bg,Bi are higher
magnification views of the areas boxed in Bb,Bd,Bf,Bh, respectively.
(Ca-Db) Downregulation of Id1, Olig2 and Nkx2.2 in Sox2+ VZ
cells between E18.5 and 22.5. Dashed lines indicate the border of the VZ. Ca
and Cb show the staining for Id1 and Sox2, respectively, in the same section.
Arrowheads show the lack of Id1 expression in some Sox2+ cells. FP,
floor plate. Scale bars: in Ac, 50 µm for
Aa-Ac,Ad,Af,Ag,Bc,Be,Bg,Bi,Bj,Cc,Cd; in Ae,Ba,Bb,Bd, 200 µm; in Bf,Bh, 100
µm; and in Cb,Db, 25 µm for Ca,Cb,Da,Db. (E) Schematic
representation of the expression patterns of patterning and
proneural/inhibitory HLH factors during the course of neuro/gliogenesis.
Colored boxes indicate the expression of Pax6 (green), Olig2 (red) and Nkx2.2
(blue) in progenitor domains. Paler colors between E16.5 and E20.5 indicate
the gradual loss of their expression in the VZ. The expression of proneural
and inhibitory HLH factors is indicated by black lines. The periods of
generation of neurons (N), oligodendrocytes (O) and astrocytes (A) are
indicated by red, blue and green lines, respectively.
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