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Fig. 3. Cell-fate specification activities of patterning and
proneural/inhibitory HLH factors. (A-H) Neurospheres subjected to
the clonal (A-D) and population (E-H) assays. The top and bottom panels in A
show the fluorescence and brightfield pictures, respectively, of
GFP+ (left) and GFP- (right) neurospheres. Arrowheads
show a virus-infected GFP+ neurosphere colony and dissociated
cells. (Ia,Ib) Effects of overexpression of patterning and HLH factors
and their combinations in the clonal assay. Viruses used for infection, either
alone (Ia) or in combination (Ib), are shown in the top row. The percentages
of various progenitor subtypes are shown in the next 11 rows of each table.
The ranges and averages of the total cell number (clone size) and numbers of
neurons and oligodendrocytes per clone (mean±s.d.) are shown in the
bottom six rows of each table. `N,' `O' and `A' stand for neurons,
oligodendrocytes and astrocytes, respectively. The data shown in red and blue
are values larger and smaller, respectively, compared with the control
culture. All data are the summary of three to four independent experiments.
*, P<0.05; **, P<0.01 compared
with the control virus; %, P<0.01 compared with Ngn2 or Ngn3
virus; $, P<0.01, compared to Mash1 virus;
&, P<0.01, compared with Id1 virus; and
#, P<0.01, compared with Hes1 virus. Scale bars: in D,
100 µm for A-D; in H, 50 µm for E-H.
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