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Fig. 5. Deficiency in oligodendrogenesis in Mash1 and Ngn2 mutants.
(Aa-De) Early generation of OLPs in various mutant mice at E12.5.
Arrowheads indicate marker-positive cells. Insets show higher magnification
views of the p* domain and adjacent areas. Intense tubular staining
in Ad-Dd and Ae-De derived from blood vessels. (E,F) Decrease in
the numbers of Olig1+ and NG2+ cells in
Mash1-/- mice between E12.5 and E16.5 compared with wild
type. The number of marker-positive cells per transverse section of the lumbar
spinal cord, including both the VZ and MZ, was quantified. Data are
mean±s.d. obtained from staining of six to ten sections derived from
two to three embryos for each genotype (*, P<0.01).
(G-M) Comparison of the numbers of cells expressing OLP markers in
various mutant mice at E12.5 (G-L) and E13.5 (M). In G and H,
Olig2+ and Nkx2.2+ cells only in the MZ were counted,
whereas in I-M, both the VZ and MZ were subjected to quantification (indicated
in boxes). Percentages are values relative to the wild type. Data are
mean±s.d. obtained from staining of eight to ten sections derived from
two to three embryos for each genotype. *, P<0.01
compared to the wild type; $, P<0.05; and
$$, P<0.01 compared to Mash1-/-
mice. Scale bars: in Dd, 100 µm for Aa-Dd; in De, 50 µm for Ae-De.
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