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Fig. 5. Synergistic effects of the ale1 and ale2 mutations on
organ formation and on the formation of leaf cuticle. (A) Scheme
for the isolation of ale1 ale2 double mutants. Approximately 25% of
seeds from ale1: ale2/+ parents were either malformed (Aa) or
shriveled (Ab). Seedlings from the malformed seeds had serious morphological
defects, to various extents (Ac-Ae). (B) Epidermal surface defects
verified with TB. Leaves of each single mutant were stained in a patchy
pattern. Most of the surface of aerial parts of the ale1 ale2 double
mutant was stained. (C) Quantification of the epidermal surface defects
in 2-week-old plants by the TB test. (D) Transmission electron
micrographs showing the surface of epidermal cells. Typical electrondense
granules found in ale1 ale2 cells are indicated by asterisks.
(E) Synergistic effects of the ale1 and ale2
mutations on shoot organization. Shoot apices of 5-day-old plants are shown.
c, cuticle; cw, cell wall; cp, cytoplasm. Scale bars: 1 µm in D; 20 µm
in E.
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