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Figure 2


Fig. 2. RNA in situ hybridisations and alteration of DR5 and PIN1 expression in drn mutants. (A-D) DRN expression on median longitudinal sections of wild-type embryos in early globular (A), late globular (B), early heart (C) and torpedo (D) stages. (E-H) DRNL expression in wild-type embryos in early globular (E), late globular (F), early heart (G) and late heart (H) stages. (I-K) DR5::GFP expression in globular wild-type (I), globular drn-1 mutant (J) and drn-1 transition-stage (K) embryos. The arrow in J marks the hypophysis region. Note supernumery cell files in the suspensor (K). (L,M) DR5::GFP expression in wild-type (L) and drn-1 (M) heart-stage embryos. Note the normal and mutant cotyledons in M. (N-Q) PIN1 expression in wild-type (N) and drn (O) 32-cell-stage embryos, and in wild-type (P) and drn (Q) globular embryos. Arrows show basal PIN1 polarity (N,P) and abnormal lateral PIN1 expression (O,Q). (R-U) RNA in situ hybridisation for PHV on median longitudinal sections of wild-type embryos at early globular (R), late globular (S), heart (T) or late heart (U) stage.





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