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Fig. 3. CoIP and BiFC analysis for the interaction between DRN or DRNL and
PHV. (A,B) Full-length PHV protein can precipitate
fulllength DRN (A) or DRNL (B). Protein mixtures were precipitated via PHV-HA.
The grid summarises the IP and Co-IP lanes and the antibodies used. Asterisks
mark the co-eluted IgG light chain. (C-E) Cellular fluorescence of
GFP-DRN (C), GFP-DRNL (D) or GFP-PHVs (E) in leek epidermal cells.
(F,G) Bimolecular fluorescence complementation showing YFP
expression observed with a GFP filter in leek epidermal cells following
co-bombardment of full-length DRN and PHVs proteins (F) or full-length DRNL
and PHVs proteins (G) fused to complementary YFP subdomains.