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Figure 1


Fig. 1. Expression patterns of DjCHC mRNA and protein. (A-C) DjCHC mRNA, analyzed by whole-mount in situ hybridization. (A) Dorsal view. a, anterior; p, posterior; Ph, pharynx. (B) DjCHC expression at 7 days after X-ray irradiation. (C) DjCHC sense probe. (D) DjCHC protein, analyzed by whole-mount immunostaining. VNCs, ventral nerve cords. (E) Higher magnification of a single section of the confocal image of the head region shown in D. (F) Schematic illustration of the head region of planarian. The planarian CNS, consisting of an inverted U-shaped spongy region (red) and nine lateral branches (green). (G) Upper panels show whole-mount in situ hybridization and lower panels show whole-mount immunostaining of planarians at 1, 2, 3, 5 or 7 days after amputation. Scale bars: 100 µm.





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