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Fig. 4. DjCHC-RNAi treatment caused CNS atrophy. (A)
DjCHC-RNAi treatment caused atrophy of the head and brain from 7 to
14 days after the injection of intact animals. (B) Numbers of
head-atrophied planarians in control, X-ray-irradiated,
DjCHC-RNAi-treated and DjCHC-RNAi plus X-ray-irradiated
planarians. n=30. (C) BrdU-labeling of the trunk piece of
control and DjCHC-RNAi-treated planarians. Samples were fixed 4 or 48
hours after BrdU treatment. a, anterior; p, posterior; ph, pharynx. (D)
Frequency of mitosis in uncut planarians detected using anti-H3P. Confocal
single projection of the fluorescent image of H3P-positive cells (magenta) and
the CNS of the identical animal visualized using anti-DjSYT (green) are shown.
Control, DjCHC-RNAi-treated and X-ray-irradiated planarians were
fixed 14 days after RNAi treatment or irradiation. Animals are oriented with
anterior towards the top. (E) The number of labeled mitoses determined
using anti-H3P in planarians. Each column represents the average number
(±s.e.m.) of anti-H3P-positive cells from 12 planarians. Similar data
were obtained in experiments using BrdU. ***P<0.01. NS,
not significant. Scale bars: 100 µm.
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