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Figure 8


Fig. 8. Clathrin-associated endocytic genes are required for proper brain regeneration. (A) Whole-mount immunostaining with anti-PC2 (Agata et al., 1998) of planarians at 7 days of regeneration treated with dsRNA of Djunc13A (AB281585), DjsbpA (AB281586) and Djsyt. (B) The phylogenetic tree was constructed by the neighbor-joining method using the full sequences. The tree includes the amino acid sequences of the mu-1 (µ1) and mu-2 (µ2) genes derived from Dugesia japonica (AB243058, AB243059), Homo sapiens (Q9BXS5, Q9Y6Q5, Q96CW1), Mus musculus (P35585, Q9WVP1, P84091), Drosophila melanogaster (EAL28715, AAF56001) and Caenorhabditis elegans (NP491572, AAA72418, P35603). (C,D) The expression pattern of Djmu-1 (Djµ1; C) and Djmu-2 (Djµ2; D). (E) Whole-mount immunostaining with anti-DjSYT of planarians at 7 days of regeneration that had been treated with RNAi for DjCHC, Djmu-1, Djmu-2, Djmu-1 plus Djmu-2, or DjCHC plus Djmu-2. The numbers in each panel represent the downregulated expression level of each gene analyzed by real-time PCR. The dashed line indicates the border between the newly formed region and the old stump region. Scale bars: 100 µm. (F) The numbers on the y axis indicate the percentage of head-atrophied planarians on each day after dsRNA treatment (x axis). n=30.





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