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Fig. 10. Comparison of petal reflexing, epidermal cell shape and the levels of
MIXTA and AmMYBML2 transcripts in petals of recently opened
buds of A. majus, A. barrelieri and A.
australe. (A-C) Freeze-fracture SEM micrographs of sections
across the mid-point of the left dorsal petal of A. majus (A), A.
barrelieri (B) and A. australe (C). The cells in A.
barrelieri are more conical than in A. majus. The cells in
A. australe are the flattest. (D-F) This is supported by
surface SEM views of these cells in A. majus (D), A.
barrelieri (E) and A. australe (F). Scale bars: 50 µm.
(G-I) The degree of reflexing of the dorsal petals is small in A.
majus (G), minimal in A. barrelieri (H) and very strong in
A. australe (I). (J) RNA gel blots of poly(A+) RNA from buds
just prior to opening of A. majus (A.m), A.
australe (A.a) and A. barrelieri (A.b),
showing the relative expression of MIXTA and AmMYBML2 in the
different species of Antirrhinum. A cDNA fragment encoding ubiquitin
was used to probe the RNA gel blots as a loading control. The ubiquitin
transcript was polymorphic in A. majus (two bands). Only the larger
transcript was detected in A. australe, whereas in A.
barrelieri only the smaller transcript was detected.
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