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Fig. 3. Comparison of expression patterns of PhMYB1 and
AmMYBML2. (A) Expression of PhMYB1 during petal
development of Petunia hybrida. RNA gel blot analysis of total RNA
from petals of increasing size (1, 0-1 cm; 2, 1-2 cm; 3, 2-3 cm; 4, 3-4 cm; 5,
open flower). The RNA was probed with a fragment corresponding to the 3'
end of the PhMYB1 gene. (B) In situ hybridisation of
PhMYB1 (antisense probe) in petals of Petunia between stages
2 and 3. The control shows hybridisation to the sense probe. Expression of
PhMYB1 was detected in both the inner and outer epidermis of the
corolla lobe. (C) Expression of AmMYBML2 in A. majus.
RNA gel blot analysis of poly(A+) RNA from petals of increasing size (1, 0-0.5
cm; 2, 0.5-1 cm; 3, 1-1.5 cm; 4, 1.5-2 cm; 5, 2-2.5 cm; 6, 2.5-3 cm), leaves
(y, young; me, medium; ma, mature) and roots, probed with the full-length
AmMYBML2 cDNA. Petal RNA was also probed with MIXTA to show
that expression of AmMYBML2 peaks later than that of MIXTA.
(D) In situ hybridisation of AmMYBML2 in petals of A.
majus at stage 4, showing weak expression in both the inner and outer
epidermal cell layers. The control shows hybridisation to the sense probe.
Scale bars: 100 µm.
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