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Figure 2


Fig. 2. YAC d16Z contains Gata2 endothelium regulatory sequences. (A-E) Embryos at E8.5 (A), E10.5 (B) and E12.5 (C-E) bearing the Gata2 d16 lacZ-tagged yeast artificial chromosome (Zhou et al., 1998) were stained for ß-galactosidase activity as whole-mount (A,C-E) or cryosectioned (B) specimens. Strong lacZ expression was observed in the developing heart tube (arrowhead, A), in the aorta and the endocardium (ec; B), in the remodeled vasculature of the yolk sac (C), and in the embryo proper (D,E), as well as in the umbilical vessels (E), where the staining superficially appeared to be weaker in the umbilical vein (uv) than in the umbilical artery (ua).





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