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Figure 4


Fig. 4. Inner ears of Gli2 and Gli3 compound mutants. (A-D) Lateral views of inner ears and (E-H) ventral views of cochlear ducts of (A,E) Gli2+/-;Gli3+/-, (B,F) Gli2-/-;Gli3+/+, (C,G) Gli2-/-;Gli3+/-, (D,H) Gli2+/-;Gli3-/- embryos at 14.75 dpc. The dorsal region of the inner ears is normal (A-C), except in Gli2+/-;Gli3-/- (D). By contrast, the length of the cochlear duct in Gli2-/-;Gli3+/- is shortened (G, arrow). (I) Bar chart showing ratios of cochlear duct lengths between compound mutants and their Gli2+/-;Gli3+/- littermate controls. Asterisk indicates an average ratio that is significantly different from others (P<0.0001; Student's unpaired t-test). Scale bar: 100 µm.





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