spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Recombinant laminin that contains dystroglycan-binding, but not integrin-binding, sites binds to the surface of differentiated oligodendrocytes. (A) rE3 is a recombinant laminin protein that comprises the last two LG domains of the laminin {alpha}-subunit. rE3 has been shown previously to contain dystroglycan-binding, but not integrin-binding, sites. Oligodendrocytes were differentiated for the indicated times in the presence or absence of 10 µg/ml rE3, washed to remove unbound protein and lysed. Western blots of lysates were performed to detect the presence of rE3. Blots were reprobed with actin antibodies as a loading control. (B) Densitometry to determine the average relative intensity of the captured rE3 signal (n=3, **P<0.01, error bars represent s.d.). (C) FLAG antibodies followed by FITC-conjugated secondary antibodies were used to detect cell-surface binding of rE3 to differentiated oligodendrocytes. Control (ctrl) image depicts FLAG immunofluorescence on cells without added rE3. Representative merged phase and fluorescent micrographs are shown.





Right arrow Return to article