spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Enhancement of survival by laminin requires ß1-integrins, but not dystroglycan. (A) Indirect immunofluorescent TUNEL to detect cell death in oligodendrocytes transfected with control (ctrl) or dystroglycan (DG) siRNA. Bar graph depicts the average percentage of TUNEL-positive cells relative to total cell population (no significant difference, n=3, error bars represent s.d.). (B) Oligodendrocyte progenitors were differentiated on poly-D-lysine (PDL) or laminin for 4 days with increasing amounts of soluble growth factor PDGF. Integrin- and dystroglycan-blocking antibodies, or control antibodies, at 10 µg/ml were added as indicated. Survival was evaluated using TUNEL on the GalC-positive cell population (newly formed oligodendrocytes). Laminin caused a significant shift in the PDGF dose-response such that survival was increased in response to 0.1 and 1.0 ng/ml PDGF compared with cells grown on PDL (**P<0.01, error bars represent s.d.). Antibodies against ß1-integrin, but not dystroglycan, blocked the ability of laminin to amplify PDGF-mediated survival at all concentrations (**P<0.01, error bars represent s.d.).





Right arrow Return to article