DEV02845 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 (Adobe Acrobat) -

  Fig. S1. Dlx2a can bind to the DLX binding site present in the I12b enhancer. In electromobility shift assays, a GST fusion protein containing zebrafish Dlx2a shows binding to a double-stranded oligonucleotide probe corresponding to the FP4 binding site (bracket).

• Supplemental Figure 2 (Adobe Acrobat) -

  Fig. S2. Identification of the conserved element E1 in the promoter region of the Hes6 gene through sequence comparison. (A) Percentage identity plot (PIP) of the Hes6 gene from mouse and human. The mouse sequence is shown on the horizontal axis and the percentage identity to the human sequence is shown on the vertical axis. Numbered black and light-gray boxes represent the four Hes6 exons with coding sequence shown in black and non-coding in gray. In the promoter region, a conserved element (E1 box) located 671 bp from the mouse Hes6 exon1 was found. Repetitive sequences are shown as follows: vertical white rectangles, simple sequence repeats; light-gray triangle, short interspersed element (SINE); dark-gray triangle and pointed boxes, long terminal repeat (LTR) and transposons; white horizontal rectangles, CpG island with CpG ratio >0.60; gray rectangles, CpG island with CpG ratio >0.75. For further details on PIP analyses see http://bio.cse.psu.edu/pipmaker. (B) Sequence alignment of the E1 enhancer from mouse (mHes6) and human (hHes6). The 217 bp element contains three conserved E-boxes (red). The boundaries of the primers used in the ChIP assay are represented by blue arrows.