spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Dlx2a can bind and activate transcription through the intergenic I12b enhancer. (A) In a transient transfection assay, zebrafish Dlx2a upregulates transcription of a CAT reporter construct containing the I12b enhancer (mI12b-pBLCAT2) by a factor of 11. By comparison, expression of a CAT reporter gene under control of the I56i enhancer (Zerucha et al., 2000) was activated 5.6-fold in the presence of Dlx2a. (B) The disruption of the Dlx2a homeodomain completely abolishes this activation. In the presence of zebrafish Dlx2a, a 9.03-fold increase in CAT activity was observed, whereas the Dlx2a homeodomain mutant showed only a basal level of CAT activity. Values shown represent the mean of relative CAT activity obtained from two to three independent experiments ± s.e.m.





Right arrow Return to article