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Figure 6


Fig. 6. Mutagenesis of protein-binding sites on the I12b enhancer affects its activity in the diencephalon. Transverse sections through the diencephalon, at the caudal limit of the telencephalon, in E11.5 transgenic mouse embryos (such as those shown in Fig. 4). The plane of section is schematized. Activity of the wild-type I12b enhancer (A) and following mutagenesis of (B) the first DLX/MSX/NKX2.5 site, (C) MEIS1 site, (D) FP3 site, (E) second DLX/MSX/NKX2.5 site, (F) MASH1 site, and (G) FP6 site. Scale bar: 500 µm.





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