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Fig. 3. kra-null mutants. (A) Genomic organization of
the kra locus. CG1427 and Vha26 genes are flanking
the kra locus at position 83B4 on the right arm of the third
chromosome. The relative positions of P-element insertions l(3)j9B6
and EP(3)0428 are indicated as inverted triangles. The extents of
deletions in kra1 and kra2 are
indicated. For the predicted transcripts, black boxes represent coding regions
and white boxes represent untranslated regions; predicted translation
initiation and stop sites are indicated. (B) Western blot analysis of
larval extracts using anti-Kra. The same blot was reprocessed with
anti-ß-actin to confirm equal protein loading. (C) RT-PCR analysis
of CG1427 and rp49 expression in third instar wild-type and
kra1/kra2 mutant larvae.