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Figure 3


Fig. 3. kra-null mutants. (A) Genomic organization of the kra locus. CG1427 and Vha26 genes are flanking the kra locus at position 83B4 on the right arm of the third chromosome. The relative positions of P-element insertions l(3)j9B6 and EP(3)0428 are indicated as inverted triangles. The extents of deletions in kra1 and kra2 are indicated. For the predicted transcripts, black boxes represent coding regions and white boxes represent untranslated regions; predicted translation initiation and stop sites are indicated. (B) Western blot analysis of larval extracts using anti-Kra. The same blot was reprocessed with anti-ß-actin to confirm equal protein loading. (C) RT-PCR analysis of CG1427 and rp49 expression in third instar wild-type and kra1/kra2 mutant larvae.





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