DEV02844 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Additional adult phenotypes associated with tup clones, and summary of topographical specificity of notum phenotypes associated with tup clones. (A) Fly bearing f tup^isl-1 clones and showing a drastically reduced right heminotum (arrowhead). This phenotype was observed in 38 out of 256 heminota. (B) High magnification of a lesion on the anterior notum of a fly with y tup^ex4 clones induced at 72-96 hours AEL. y tup^ex4 microchaetae (arrows) are found within and near the lesion. Arrowhead points at two presumably y tup^ex4 sensilla trichoidea. This type of sensilla were observed in 14 out of 80 malformations, and are never found in this region of wild-type nota. (C) Graphic summary of the relative frequencies of some cuticular phenotypes in different regions of the notum of flies bearing tup clones. Green shades indicate areas of appearance of cuticular phenotypes with clear indications of notum to hinge transformations: dark green, highest frequency of appearance of ectopic tegulae; medium green, intermediate frequency of appearance of tegula-like structures, and occasional presence of ectopic sclerites or invaginations with hinge-like sensilla; light green, low frequency of appearance of tegula-like structures or cuticular lesions with hinge-like sensilla. Dark and light grey indicate high and low frequency, respectively, of appearance of cuticular malformations showing no indications of notum to hinge transformations. The data used correspond to the phenotypic analysis summarized in Table S1, plus the flies with large numbers of clones prepared as described in Materials and methods and in footnote ‡ of Table S1.

• Supplemental Figure 2 -

  Fig. S2. Some phenotypes associated with Chip and Ssdp clones. (A) Second-instar-induced Chip clones (absence of green). Zfh2 (red) is detected only in the clone located within the tup expression domain. (B) Lateral view of the notum of a fly in which Chip unmarked clones have been induced (anterior is to the left). Arrows indicate ectopic tegula (tg′) on the notopleural region, with mirror symmetry with respect to the extant tegula (tg). Ectopic tegula possessed sensilla campaniformia and trichoidea (not shown). Arrowheads indicate a depigmented region of cuticle surrounding the ectopic tegula; depigmentation is associated with many tup clones. (C) Dorsal view of a fly in which Ssdp^neo48 clones were induced (anterior is to the top). A cuticular lesion appears in the lateral, posterior notum, bearing sensilla trichoidea (brackets). These are shown at higher magnification in the inset beneath. (D) Outgrowth (white rectangle) on the anterior lateral notum of a fly in which Ssdp^neo48 clones were induced (anterior is to the top). Arrow indicates extant proximal costa. (E) High magnification of the outgrowth shown in D. The presence of bracts (arrowheads) associated with the bristles identifies the outgrowth as ectopic proximal costa (Bryant, 1975).

  Reference

  Bryant, P. J. (1975). Pattern formation in the imaginal disc of Drosophila melanogaster: fate map, regeneration and duplication. J. Exp. Zool. 193, 49-78.