DEV02837 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Normal migration and distribution of neural crest cells in Snai1/2-dko mutant embryos. Snai1^flox/flox Snai2^+/− mice were bred to a ROSA26-EGFP reporter in order to mark neural crest cells with the Wnt1-Cre driver. (A-D) Normal distribution of GFP-labeled cells in Snai1/2-dko embryos at E11.5. (E-J) Anti-GFP immunostaining demonstrating no alterations in the distribution of neural crest cells in the developing palate at E12.5. Note that the staining apparent in the mesenchyme is excluded from the epithelium, demonstrating the specificity of the antibody. (K-P) E14.5 embryos. Scale bar: 100 μm.

• Supplemental Figure 2 -

  Fig. S2. Histological sections of the anterior palatal shelves of control and Snai1/2-dko embryos. Hematoxylin and Eosin staining of E13.5 control (A,C,E) and mutant (B,D,F) heads sectioned at equivalent planes. Note that some pairs appear very similar in the degree of palate shelf outgrowth (A,B), whereas some control examples show a greater degree of outgrowth at this stage (C, arrow). At a slightly more posterior plane, the palatal shelves are consistently similar in the degree of outgrowth (E,F). Scale bar: 200 μm.